Expt # | Culture system | PBMC seeded per vessel | Average floating cells recovered | Average mature DC (CD83+, CD86hi) recovered | % yield of mature DC |
---|---|---|---|---|---|
1** | 850 cm2 roller bottles | 900 × 106 | 106 × 106 | 85 × 106 | 9.4% |
T-175 static flasks | 180 × 106 | 20 × 106 | 19.2 × 106 | 10.7% | |
2** | 850 cm2 roller bottles | 900 × 106 | 100 × 106 | 82 × 106 | 9.1% |
T-175 static flasks | 180 × 106 | 16 × 106 | 10.2 × 106 | 5.7% | |
3*** | 850 cm2 roller bottles | 800 × 106 | 84 × 106 | 23 × 106 | 2.9% |
T-175 static flasks | 200 × 106 | 29 × 106 | 6 × 106 | 3% |