Journal of Immune Based Therapies and Vaccines

Table 5 Cytokine levels for various peptide formulations by cocktail approach. Cytokine levels as measured for various peptide formulations generated by cocktail approach (The value represents the level of cytokines obtained from wells pulsed with the optimal antigen (peptides alone or in adjuvant systems) concentration which showed maximal T-cell stimulation index for mice primed with cocktail of peptides.

From: Generating neutralizing antibodies, Th1 response and MHC non restricted immunogenicity of HIV-I env and gagpeptides in liposomes and ISCOMs with in-built adjuvanticity

Strain→	H-2 Haplotypes		Cytokines (pg/ml)
Formulation↓	BALB/c (H-2^d)	CBA/J (H-2^k)
V3-gp41 Peptide	623 ± 53	504 ± 29	IFN-γ
	440 ± 19	291 ± 38	IL-2
	41 ± 8	35 ± 4	IL-4
V3-gp41 in Liposomes	1100 ± 101	992 ± 63	IFN-γ
	487 ± 21	321 ± 28	IL-2
	70 ± 14	30 ± 2	IL-4
V3-gp41 in ISCOMs	1543 ± 165	1312 ± 287	IFN-γ
	511 ± 28	360 ± 14	IL-2
	60 ± 20	42 ± 9	IL-4
V3-gp41: MA729 in Liposomes	1210 ± 84	1413 ± 152	IFN-γ
	489 ± 31	392 ± 30	IL-2
	58 ± 2	58 ± 6	IL-4
gp41 Peptide	589 ± 54	623 ± 82	IFN-γ
	401 ± 16	368 ± 41	IL-2
	62 ± 11	48 ± 21	IL-4
gp41 Peptide in Liposomes	891 ± 16	924 ± 72	IFN-γ
	519 ± 2	400 ± 21	IL-2
	18 ± 54	32 ± 11	IL-4
gp41 Peptide in ISCOMs	937 ± 20	1321 ± 91	IFN-γ
	521 ± 9	500 ± 48	IL-2
	61 ± 156	51 ± 4	IL-4
gp41 : MA729 in Liposomes	1230 ± 19	1601 ± 134	IFN-γ
	620 ± 10	635 ± 178	IL-2
	20 ± 71	40 ± 2	IL-4
p17 Peptide	453 ± 31	368 ± 28	IFN-γ
	301 ± 31	371 ± 18	IL-2
	16 ± 3	20 ± 4	IL-4
p17 Peptide in Liposomes	1211 ± 96	892 ± 44	IFN-γ
	520 ± 25	421 ± 28	IL-2
	65 ± 9	30 ± 5	IL-4
p17 Peptide in ISCOMs	1135 ± 157	1031 ± 45	IFN-γ
	520 ± 25	423 ± 21	IL-2
	45 ± 5	31 ± 8	IL-4
p17 : MA729 in Liposomes	933 ± 28	1214 ± 76	IFN-γ
	581 ± 34	408 ± 16	IL-2
	53 ± 8	55 ± 7	IL-4
p24 Peptide	411 ± 40	284 ± 33	IFN-γ
	387 ± 22	258 ± 19	IL-2
	48 ± 7	54 ± 9	IL-4
p24 Peptide in Liposomes	989 ± 107	1123 ± 49	IFN-γ
	460 ± 42	270 ± 29	IL-2
	55 ± 6	34 ± 9	IL-4
p24 Peptide in ISCOMs	1007 ± 129	1290 ± 121	IFN-γ
	508 ± 16	689 ± 43	IL-2
	49 ± 8	36 ± 2	IL-4
p24 : MA729 in Liposomes	1241 ± 68	1334 ± 59	IFN-γ
	500 ± 24	621 ± 26	IL-2
	52 ± 7	41 ± 4	IL-4

The value represents the level of cytokines obtained from wells pulsed with the optimal antigen concentration (peptides alone or in adjuvant systems) which showed maximal T-cell stimulation index for mice primed with cocktail of peptides.

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