Identification of proteases employed by dendritic cells in the processing of protein purified derivative (PPD)

Table 1 Protease Profiles Expressed by Several DC Populations

Protease	XS52 DC	Splenic DC	Bone Marrow DC	BW5147 Thymoma Cells
Cathepsin B¹	133 ± 39²	123 ± 3.3	121 ± 3.3	1.5 ± 0.08
Cathepsin C	34 ± 7	16 ± 4	0.4 ± 0.1	<0.01
Cathepsin D/E	34 ± 6	30 ± 14	22 ± 2	1.6 ± 0.1
Cathepsin H	2.8 ± 0.8	3.5 ± 0.7	1 ± 0.2	0.9 ± 0.2
Cathepsin J	26 ± 0.3	0.7 ± 0.3	3.0 ± 0.1	0.2 ± 0.09
Cathepsin L	14 ± 0.6	26 ± 11	19 ± 0.6	0.6 ± 0.08
BLT esterase	25,000 ± 400	58,000 ± 4,000	21,300 ± 100	<100
Suc-FLF-SBz esterase	1,900,000 ± 61,000	310,000 ± 10,800	1,150,000 ± 10,200	12,000 ± 100
Suc-AAPFSBZ esterase	433,000 ± 2,900	134,000 ± 7,300	360,000 ± 6,100	3,400 ± 600

¹Extracts prepared from the indicated cell types were examined for protease activities using a panel of standard substrates. ²Enzymatic activities are expressed as nmol/min/mg soluble protein. Data shown represent the mean ± SD from three independent preparations.