Table 1 Isotype specific antibodies and streptavidin-PE used in the HPV antibody isotyping assays
Isotype Reagent | Type or MAb Identity | Specificity | Source | Supplier | Concentration |
|---|---|---|---|---|---|
Primary | |||||
IgM | polyclonal | Monkey IgM (mu chain specific) | Goat | Rockland, Gilbertsville, PA | 50 μg/mL |
IgA | polyclonal | Monkey IgA (alpha chain specific) | Goat | Rockland, Gilbertsville, PA | 25 μg/mL |
IgE | HP-6029 | Human IgE (epsilon chain specific) | Mouse | Southern Biotech, Birmingham, AL | 25 μg/mL |
IgG1 | HP-6091or 8c/639 | Human IgG1 | Mouse | Sigma, St. Louis, MO | 10 μg/mL |
IgG2 | HP-6014 | Human IgG2 | Mouse | Sigma, St. Louis, MO | 10 μg/mL |
IgG4 | HP-6025 | Human IgG4 | Mouse | Sigma, St. Louis, MO | 25 μg/mL |
Total IgG | polyclonal | Monkey IgG (gamma chain specific) | Goat | Rockland, Gilbertsville, PA | 5 μg/mL |
Secondary | |||||
PE- Streptavidin | NA | NA | NA | Rockland, Gilbertsville, PA | 5 μg/mL |