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Figure 2 | Journal of Immune Based Therapies and Vaccines

Figure 2

From: Rapid construction of a dendritic cell vaccine through physical perturbation and apoptotic malignant T cell loading

Figure 2

DC maturation induced after column separation and overnight incubation. Fig. 2A: CTCL cells and monocyte/DC isolated as described in Figure 1 were fixed and permeabilized and stained with CD36-FITC (membrane) and CD83-PE (cytoplasm). The results show 2-color quadstats gated on the monocyte population of cells obtained from a: Day 0, primary isolation; after overnight culture of b: leukapheresis cells; c: CD4-magnetic bead isolation and re-addition to column activated monocytes; d: CD3-magnetic bead purification and re-addition to column activated monocytes; e: Bar graph of the MFI of membrane CD36 expression on the cell populations. Fig. 2B: Demonstration of cytoplasmic CD83 expression in the monocyte/DC population gated by side-scatter (SS) on 100% of the monocyte population. Cell treatment a–d as described for Fig 2A.

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