Figure 3

Increased class II expression on semi-mature DC after ingestion of apoptotic CTCL cells. Fig. 3A: CTCL cells and DC prepared as described in Figure 1 were fixed and permeabilized and stained with DR-FITC (anti-class II MHC antibody, membrane) and APO2-PE (cytoplasm). The results present 2-color quadstats gated on the monocyte population of cells obtained from a: Day 0, primary isolation; b: leukapheresis cells; c: CD4-magnetic bead isolation and re-additon to column activated monocytes; d: CD3-magnetic bead purification and re-addition to column activated monocytes. Fig. 3B: Membrane DR staining on the monocyte/DC population gated on the total monocyte population by SS. Cell treatment a–d as described for Fig 3A.