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Figure 7 | Journal of Immune Based Therapies and Vaccines

Figure 7

From: Rapid construction of a dendritic cell vaccine through physical perturbation and apoptotic malignant T cell loading

Figure 7

Exogenous cytokines increase DC maturation induced after column separation and overnight incubation. Monocyte/DC populations isolated as described in Figure 1 were fixed and permeabilized and stained for expression of membrane CD36-FITC and cytoplasmic CD83-PE. The results present 2-color quadstats gated on the monocyte population of cells obtained from a: Day 0, primary isolation; b: CD3-magnetic bead purification and re-addition to column activated monocytes; c: the same CD3 column purified and activated recombined cell population cultured with the cytokines GM-CSF and IL4. Demonstration of cytoplasmic CD83 expression in the monocyte/DC population gated by side-scatter on 100% of the monocyte poulation. d: Day 0, primary isolation; e: CD3-magnetic bead purification and re-addition to column activated monocytes; f: the same CD3 column purified and activated recombined cell population cultured with cytokines.

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Journal of Immune Based Therapies and Vaccines

ISSN: 1476-8518