Figure 8

Exogenous cytokines enhance ingestion of apoptotic material in differentiating DC. CTCL cells and monocyte/DC populations isolated as described in Figure 1. The cells were fixed and permeabilized and stained for expression of membrane DR-FITC and cytoplasmic APO2-PE. The results present 2-color quadstats gated on the monocyte population of cells obtained from a: Day 0, primary isolation; b: CD3-magnetic bead purification and re-addition to column activated monocytes; c: the same CD3 column purified and activated recombined cell population cultured with the cytokines GM-CSF and IL4.