Figure 9From: Rapid construction of a dendritic cell vaccine through physical perturbation and apoptotic malignant T cell loadingMixed leukocyte culture response of normal T cells to monocytes or column activated loaded and non-loaded DC. Normal CD4 T cells were magnetic bead column purified from the peripheral blood of 2 controls and stimulated with column effluent monocytes that had been γ-irradiated to prevent differentiation, or γ-irradiated CTCL cell monocytes that had been column purified and either loaded or not with apoptotic malignant T cells and cultured overnight one day prior to the normal T cell isolation. The results are presented as delta CPM (less background proliferation obtained by autostimulation) of 3 [H]-thymidine incorporation measured at day 6. Significance was evaluated with a student's t test.Back to article page