Inhibition of CPT induces increased cell surface Fas expression. Levels of cell surface Fas on B16F1 melanoma cells in cultures with different concentrations of Etomoxir for 24 hours. Etomoxir blocks the mitochondria from using carbons from fat as fuel. Fas levels, normally low in melanoma cells, rise in the cells treated with Etomoxir. At a concentration of 500 μg/mL all the cells died. The level of Fas was detected using PE-conjugated anti-mouse Fas (CD95) antibodies (Pharmingen, California) and measured using a Coulter Elite Epics Flow Cytometer (Coulter, Hialeah, Florida) and FlowJo analysis software (Tree Star, Inc, Oregon).