Figure 3

Recogniser and Mimic peptides presented by iDCs can stimulate CD14^- PBMCs to secrete IFN-γ secretion. A. IL-12 production by immature dendritic cells. iDCs (+DCs; black bars) were incubated with or without LPS and either DSP, WHK or TMG peptides for 24 hrs. as labeled. The concentration of IL-12 (pg/mL) in the supernatants was determined by ELISA. IL-12 production in the absence of iDCs was also determined. B. iDCs were incubated with WHK, DSP or TMG peptides. Subsequently, either peptide-loaded iDCs (+iDCs) or the free peptide in solution (-iDCs), were incubated with CD14^- PBMCs from the same donor [First stimulation; (1) open bars]. These cells were incubated with a second batch of iDCs loaded with the same peptide [Second stimulation (2); filled bars]. The concentration of IFN-γ (pg/mL) in the supernatants of the CD14^- PBMCs was determined following the first (1) and second (2) stimulations.