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Figure 6 | Journal of Immune Based Therapies and Vaccines

Figure 6

From: A novel method to identify and characterise peptide mimotopes of heat shock protein 70-associated antigens

Figure 6

iDCs loaded with Hsp70 or Hsp70-PC from MDA-MB-231 cells can prime human lymphocytes to respond to synthetic DSP peptide. IDCs from two healthy female donors (Donor A; grey bars. Donor B; black bars), were incubated with either Hsp70 or Hsp70-PCs from MDA-MB-231 cells or the synthetic DSP peptide. The iDCs were then incubated with CD14- PBMCs from the same donor. [First stimulation (1)]. The cells were cultured for 10 days and then, incubated with a second batch of iDCs loaded with the synthetic peptide DSP [Second stimulation (2)]. The supernatants were assayed for IFN-γ production (pg/mL). The label 'Hsp70-PC(1)/DSP(2)' refers to IFN-γ production by CD14-PBMCs following first and second stimulations with iDCs loaded with Hsp70-PC and the DSP peptide respectively. Other labels in the figure follow a similar paradigm.

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