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Table 1 Recovery of E. coli from peritoneal fluid obtained from mice challenged with live E. coli

From: Phytol-based novel adjuvants in vaccine formulation: 2. assessment of efficacy in the induction of protective immune responses to lethal bacterial infections in mice

Bacterial challenge (CFU) 106 107 108
Time 36 hrs post infection 18 hrs post infection 36 hrs post infection 18 hrs post infection 36 hrs post infection
1Control group (non-immunized) 0 2N/A 2N/A 2N/A 2N/A
1PBS 0 3.2 ± 1.1 × 104 4.4 ± 1.2 × 105 2N/A 2N/A
1IFA 0 1.8 ± 0.5 × 104 6.0 ± 1.6 × 104 2N/A 2N/A
1PHIS-01 0 0 0 1.1 ± 0.1 × 104 0
1PHYTOL 0 0 0 2.1 ± 0.5 × 104 0
  1. 1 Peritoneal fluid samples from all groups of mice were diluted in LB medium (1:200), and 10 μl of diluted samples were streaked on agar plates using calibrated loops. CFUs were scored 18 and 36 hours following bacterial challenge and recorded as CFU/ml. The results represent the average of two experiments (n = 4 for each group) ± SEM. 2 N/A: Not assessable since all mice were dead within 18 hrs.
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