Phytol-based novel adjuvants in vaccine formulation: 2. assessment of efficacy in the induction of protective immune responses to lethal bacterial infections in mice

Table 1 Recovery of E. coli from peritoneal fluid obtained from mice challenged with live E. coli

Bacterial challenge (CFU)	10⁶	10⁷		10⁸
Time	36 hrs post infection	18 hrs post infection	36 hrs post infection	18 hrs post infection	36 hrs post infection
¹Control group (non-immunized)	0	²N/A	²N/A	²N/A	²N/A
¹PBS	0	3.2 ± 1.1 × 10⁴	4.4 ± 1.2 × 10⁵	²N/A	²N/A
¹IFA	0	1.8 ± 0.5 × 10⁴	6.0 ± 1.6 × 10⁴	²N/A	²N/A
¹PHIS-01	0	0	0	1.1 ± 0.1 × 10⁴	0
¹PHYTOL	0	0	0	2.1 ± 0.5 × 10⁴	0

¹ Peritoneal fluid samples from all groups of mice were diluted in LB medium (1:200), and 10 μl of diluted samples were streaked on agar plates using calibrated loops. CFUs were scored 18 and 36 hours following bacterial challenge and recorded as CFU/ml. The results represent the average of two experiments (n = 4 for each group) ± SEM. ² N/A: Not assessable since all mice were dead within 18 hrs.