Figure 4

Demonstration of splenomegaly in mice treated with different adjuvants. Groups of 3–4 BALB/c mice were intraperitoneally injected with each adjuvant, and after 5 days their spleens were dissected out for observation. A representative result is shown below: (A) Effects ofvarious adjuvants on spleen size: 1. Spleens from mice injected with PBS. 2. Spleen from mice injected with Pristane. 3. Spleen from mice injected with IFA. 4. Spleen from mice injected with CFA. 5. Spleen from mice injected with Phytol. (B) Effects of different doses of phytols on spleens: 1. Spleen from mice injected with 100 μl of PBS. 2. Spleen from mice injected with 80 μg of Phytol. 3. Spleen from mice injected with 40 μg of Phytol. 4. Spleen from mice injected with 80 μg of PHIS-01. 5. Spleen from mice injected with 40 μg of PHIS-01