Table 3 Ascites production from syngeneic BALB/C mice using various priming agents
Hybrid line | Isotype of Ig | 1Priming agent | Total volume collected (ml) | Mouse number providing ascites/number injected | 2Yield/mouse (ml) | 3Antibody titer (OD @ 490 nm) |
|---|---|---|---|---|---|---|
2C3 | IgG1 (γ1, κ) | None (PBS) | None | 0/4 | None | 4ND |
| Â | Â | Pristane | 12 | 4/4 | 3 | 1.3 |
| Â | Â | Phytol | 1.6 | 1/4 | 1.6 | 1.1 |
| Â | Â | PHIS-01 | 8 | 3/4 | 2.7 | 0.9 |
1H524 | IgM (μ, κ) | None (PBS) | None | 0/4 | None | 4ND |
| Â | Â | Pristane | 16 | 4/4 | 4 | 0.8 |
| Â | Â | Phytol | 9.3 | 2/4 | 4.65 | 0.75 |
2B424 | IgM (μ, κ) | None (PBS) | None | 0/4 | None | 4ND |
| Â | Â | Pristane | 15 | 3/4 | 5 | 1.15 |
| Â | Â | Phytol | 19 | 4/4 | 4.75 | 1.32 |