Table 1 Monoclonal antibodies used to test the expression of cell surface markers of monocyte-derived macrophages and dendritic cells stimulated or not with CpG-ODN or LPS
From: The effect of CpG-ODN on antigen presenting cells of the foal
MARKER | ANTIBODY | CLONE | SUPPLIER | VALIDATION |
|---|---|---|---|---|
CD172a | mouse anti-bovine CD172a | DH59B | VMRD, Pullman, WA | Kydd et al., 1994 |
CD86 | mouse anti-human CD86 | 2331(FUN-1) | Becton and Dickinson, San Diego, CA | Hammond et al., 1999 |
MHC I | mouse anti-horse MHC I | CZ3 | D. Antczak's laboratory, Cornell University | Lunn et al., 1998 |
MHC II | mouse anti-horse MHC II | CZ11 | D. Antczak's laboratory, Cornell University | Lunn et al., 1998 |
CD14 | mouse anti-human CD14 | big10 | Biometec, Germany | Steinbach et al., 1998 |
Negative | mouse anti-canine parvovirus | -- | C.Parrish's laboratory, Cornell University | Parrish et al., 1982 |