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Table 2 Primer and probe sequences used to measure mRNA expression in monocyte-derived macrophages and dendritic cells

From: The effect of CpG-ODN on antigen presenting cells of the foal

CYTOKINE PRIMER AND PROBE SEQUENCES GenBank accession #
IL-12p35 5'-TCA AGC TCT GCA TCC TTC TTC AT-3' Y11130
  5'-CAG ATA GCC CAT CAT CCT GTT G-3'  
  5'-FAM-CCT TCA GAA TCC GCG CAG TGA CCA-TAMRA-3'  
IL-12p40 5'-CAC CTG CAA TAC CCC TGA AGA-3' Y11129
  5'-TGC CAG AGC CTA AGA CCT CAT T-3'  
  5'-FAM-CAT CAC CTG GAC CTC GGC CCA-TAMRA-3'  
IFNα 5'-AGG TGT TTG ACG GCA ACC A-3' M14540
  5'-ACG AGC CGT CTG TGC TGA A-3'  
  5'-FAM-AGC CTC AAG CCA TCT CCG CGG T-TAMRA-3'  
IL-10 5'-GAC ATC AAG GAG CAC GTG AAC TC-3' U38200
  5'-CAG GGC AGA AAT CGA TGA CA-3'  
  5'-FAM-AGC CTC ACT CGG AGG GTC TTC AGC TT-TAMRA-3'  
TNFα 5'-GAT GAC TTG CTC TGA TGC TAA TCC-3' M64087
  5'-TCT GGG CCA GAG GGT TGA T-3'  
  5'-FAM-TCT CCC CAG CAG TTA CCG AAT GCC TT-TAMRA-3'  
TLR9 5'-AAC TGG CTG TTC CTG AAG TCT GTG-3' DQ157779
  5'-TCA ACC TCA AGT GGA ACT GCC C-3'  
  5'-FAM-AGA GAA CTG TCC TTC AAC ACC AGG-TAMRA-3'  
β-actin 5'-TCA CGG AGC GTG GCT ACA-3' AF035774
  5'-CCT TGA TGT CAC GCA CGA TTT-3'  
  5'-FAM-CAC CAC CAC GGC CGA-TAMRA-3'