Table 2 Primer and probe sequences used to measure mRNA expression in monocyte-derived macrophages and dendritic cells
From: The effect of CpG-ODN on antigen presenting cells of the foal
CYTOKINE | PRIMER AND PROBE SEQUENCES | GenBank accession # |
|---|---|---|
IL-12p35 | 5'-TCA AGC TCT GCA TCC TTC TTC AT-3' | Y11130 |
| Â | 5'-CAG ATA GCC CAT CAT CCT GTT G-3' | Â |
| Â | 5'-FAM-CCT TCA GAA TCC GCG CAG TGA CCA-TAMRA-3' | Â |
IL-12p40 | 5'-CAC CTG CAA TAC CCC TGA AGA-3' | Y11129 |
| Â | 5'-TGC CAG AGC CTA AGA CCT CAT T-3' | Â |
| Â | 5'-FAM-CAT CAC CTG GAC CTC GGC CCA-TAMRA-3' | Â |
IFNα | 5'-AGG TGT TTG ACG GCA ACC A-3' | M14540 |
| Â | 5'-ACG AGC CGT CTG TGC TGA A-3' | Â |
| Â | 5'-FAM-AGC CTC AAG CCA TCT CCG CGG T-TAMRA-3' | Â |
IL-10 | 5'-GAC ATC AAG GAG CAC GTG AAC TC-3' | U38200 |
| Â | 5'-CAG GGC AGA AAT CGA TGA CA-3' | Â |
| Â | 5'-FAM-AGC CTC ACT CGG AGG GTC TTC AGC TT-TAMRA-3' | Â |
TNFα | 5'-GAT GAC TTG CTC TGA TGC TAA TCC-3' | M64087 |
| Â | 5'-TCT GGG CCA GAG GGT TGA T-3' | Â |
| Â | 5'-FAM-TCT CCC CAG CAG TTA CCG AAT GCC TT-TAMRA-3' | Â |
TLR9 | 5'-AAC TGG CTG TTC CTG AAG TCT GTG-3' | DQ157779 |
| Â | 5'-TCA ACC TCA AGT GGA ACT GCC C-3' | Â |
| Â | 5'-FAM-AGA GAA CTG TCC TTC AAC ACC AGG-TAMRA-3' | Â |
β-actin | 5'-TCA CGG AGC GTG GCT ACA-3' | AF035774 |
| Â | 5'-CCT TGA TGT CAC GCA CGA TTT-3' | Â |
| Â | 5'-FAM-CAC CAC CAC GGC CGA-TAMRA-3' | Â |