Figure 2

DC differentiation from monocytes induced by column passage and apoptotic CTCL cell loading. DC and apoptotic CTCL cells were co-cultured following passage of MNL through an anti-CD3 magnetic bead column. DC differentiation was monitored on Day 1 by measuring expression of cell surface markers using a flow cytometer gated on the monocyte population. A. Significant decrease in expression of monocyte marker CD14 was found on column-passaged monocytes co-cultured with apoptotic CTCL cells when compared to the level identified on Day 0 monocytes. B. Significant increase in expression of class II was found on column-passaged monocytes co-cultured with apoptotic CTCL cells when compared to the level identified on Day 0 monocytes. C. Significant increase in the expression of CD86 was found on column-passaged monocytes co-cultured with apoptotic CTCL cells when compared to the level identified on Day 0 monocytes Statistical analysis performed with student's t test or Mann Whitney Rank-Sum Test. Apoptotic = CD3-treated, Viable = CD4-treated, NS = not significant