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Figure 4 | Journal of Immune Based Therapies and Vaccines

Figure 4

From: Improved generation of anti-tumor immunity by antigen dose limitation

Figure 4

Dose-response curve of Treg induction in CTCL cells. A. Increasing numbers of CD3-treated apoptotic CTCL cells (0.3 × 106, 1.5 × 106, 3.0 × 106) were added to a constant number of DC (105), co-cultured overnight and used to stimulate freshly isolated autologous CTCL cells. As controls, increasing numbers of non-loaded DC were cultured with CD4-treated viable CTCL cells. Then additional CTCL cells were added and co-cultured overnight. The percentage of membrane CD3+/cytoplasmic CTLA-4+ CTCL cells was measured by flow cytometry gated on the lymphocyte population. B. Increasing numbers of apoptotic CTCL cells (0.10 × 106, 0.5 × 106, 1.0 × 106) were added to 105 DC and co-cultured overnight, followed by addition of 106 purified CTCL cells. The percentage of membrane CD4+/cytoplasmic FoxP3+ expressing cells was measured by flow cytometry. Apoptotic = CD3-treated, Viable = CD4-treated

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