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Figure 5 | Journal of Immune Based Therapies and Vaccines

Figure 5

From: Improved generation of anti-tumor immunity by antigen dose limitation

Figure 5

Dose-dependent decrease in CD8-perforin expression as number of phenotypic Treg cells increase. A. Increasing numbers of CD3-treated apoptotic CTCL cells (0.3 × 106, 1.5 × 106, 3.0 × 106) were added to a constant number of DC (105), co-cultured overnight and used to stimulate freshly isolated autologous CTCL cells and CD8+ cells. As the percentage of membrane CD3+/cytoplasmic CTLA4+ cells increased there was an inversely related decrease in the number of membrane CD8+/cytoplasmic perforin+ cells. B. Increasing numbers of apoptotic CTCL cells (0.10 × 106, 0.5 × 106, 1.0 × 106) were added to 105 DC and co-cultured overnight, followed by addition of 106 purified CTCL cells and CD8+ cells. As the percentage of membrane CD4+/cytoplasmic FoxP3+ cells increased, there was an inversely related decrease in the number of membrane CD8+/cytoplasmic perforin+ cells. Apoptotic = CD3-treated, Viable = CD4-treated

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