Figure 6

Expansion of CD8 T cell compartment following co-culture with DC loaded with low numbers of apoptotic CTCL cells. A. Autologous DC (10⁶) were co-cultured with 1.0 × 10⁶ and 0.25 × 10⁶ apoptotic CTCL cells, followed by addition of freshly isolated autologous CTCL cells and CD8⁺ cells. Percentage of CD8⁺ T cells in co-culture increased significantly upon co-culture with autologous DC loaded with 0.25 × 10⁶ apoptotic cells. B. The absolute number of CD8⁺ T cells increased upon co-culture with DC loaded with 0.25 × 10⁶ apoptotic cells. Data analyzed using student's t test. Apoptotic = CD3-treated, Viable = CD4-treated, NS = Not significant.