Figure 2

Reproducibility of T cell responses to CMV pp65 and SEB. Frozen PBMCs from 3 donors were independently thawed and cultured with CMV pp65 peptide pool or vehicle (panels A and B) or with SEB (panels C and D) by two operators at five different occasions. The expression of IL-2, INF-γ and TNF-α in CD3⁺CD4⁺ and CD3⁺CD8⁺ cells was determined by specific staining and flow cytometry analysis. Background cytokine expression from unstimulated cells was subtracted from CMV pp65 peptides-stimulated cells. Percentages of CD4⁺ (panel A and C) and CD8⁺ (panel B and D) T cells either IL-2^- IFN-γ⁺ TNF-α^-, IL-2^- IFN-γ^- TNF-α⁺, IL-2⁺ IFN-γ^- TNF-α^-, IL-2^- IFN-γ⁺ TNF-α⁺, IL-2⁺ IFN-γ^- TNF-α⁺, IL-2⁺ IFN-γ⁺ TNF-α^- or IL-2⁺ IFN-γ⁺ TNF-α⁺ obtained for the three donors in ten independent experiments are presented.