Effect of maturation on MDDC derived from fresh PBMC of healthy donors (Fresh Healthy), cryopreserved PBMC of healthy donors (Cryo. Healthy), and cryopreserved PBMC of cancer patients (Cryo. Cancer). A. Surface phenotype: Immature and mature MDDC from each of the three groups were compared for their expression levels (MFI) of CD86, CD83, and HLA-DR. B. Function: Immature and mature MDDC were cultured for additional 18–20 h in presence of BFA. Cells were processed and analyzed to evaluate the expression of intracellular IL-12 (% CD209+IL-12+) or COX-2 (% CD209+COX-2+). Quantities of secreted IL-8 by immature and mature MDDC from each of these two groups were detected by CBA assay of the culture supernatants collected on day 7. Bars in all the scatter plots represent medians. **, statistically significant differences (P < 0.01); ***, statistically significant differences (P < 0.001).