Figure 4From: Phenotype and in vitrofunction of mature MDDC generated from cryopreserved PBMC of cancer patients are equivalent to those from healthy donorsComparison of mature MDDC derived from cryopreserved PBMC of healthy donors vs. cancer patients. A. Surface phenotype: Expression levels (MFI) of CD86, CD83, and HLA-DR on mature MDDC derived from healthy donors (healthy) were compared to those derived from cancer patients (cancer). B. Function: Mature MDDC from each group were cultured for additional 18–20 h in presence of BFA. Cells were processed and analyzed to evaluate the expression of intracellular IL-12 (%CD209+IL-12+) or COX-2 (%CD209+COX-2+) as described earlier. Quantities of secreted IL-8 (pg/ml) by mature MDDC from each of these two groups were detected by CBA assay of the culture supernatants collected on day 7. C. T cell stimulation: The top scatter plot shows proliferation of allogeneic CD4+ T cells using mature MDDC from PBMC of healthy donors and cancer patients. The lower two scatter plots demonstrate enhancement of MDDC mediated SEB-specific autologous CD4+ and CD8+ T cell proliferation. Both allogeneic and autologous antigen-specific T cell stimulation assays were set up and percent proliferation was measured as described earlier. Bars in all the scatter plots represent medians. *, statistically significant differences (P < 0.05); **, statistically significant differences (P < 0.01).Back to article page