Enhancement of SEB-specific proliferation of autologous CD4+ T cells by mature MDDC. Histograms in this figure show the CFSE staining profile of CD4+ T cells from cryopreserved PBMC stimulated with autologous DC pulsed with SEB (A) data from a representative healthy donor, and (B) data from a representative cancer patient. Proliferation of CD4+ T cells in presence of SEB alone was 3.1% (healthy donor) and 0.35% (cancer patient). Proliferation is measured as the percentage of cells showing decreased staining intensity of CFSE compared to the intensity of the CFSEbright population (marked as Peak 1 in all histograms). Numbers in all histograms represent %proliferation.