Figure 3

Enhanced DC elimination during DC vaccination combined with CTLA-4 blockade. C57/Bl6 mice were immunized with peptide LCMV gp33_33–41 loaded DCs with or without i.p. injection of anti-CTLA-4 mAb. After 7–10 days, an inoculum consisting of peptide LCMV _33–41 loaded CFSE labeled together with peptide unloaded CMTMR labeled DCs was injected subcutaneously into the distal forelimb of naïve mice (control), immunized mice or immunized mice cotreated with anti-CTLA-4 mAb. DCs were recovered from the draining lymph node for FACS analysis and determination of % surviving DCs. Results are shown as mean ± SD of nine mice from 3 separate experiments. (* p < 0.05; ***p < 0.0001)