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Figure 3 | Journal of Immune Based Therapies and Vaccines

Figure 3

From: A new approach for the large-scale generation of mature dendritic cells from adherent PBMC using roller bottle technology

Figure 3

Roller bottle cultures yield mature CD83+ DC with high viability. Mature mDC were generated in 850 cm2 roller bottles or in T-175 static flasks as before using ITIP maturation. The floating cells were harvested and stained for DC maturation markers without fixation. Immediately before FACS analysis 2 μg/ml 7-ADD was added as viability indicator. The dot plots shown the total cells in the floating fractions with the CD83+, 7-AAD- and CD83+, 7-AAD+ cells gated. In both cases, the CD83+ cells exhibited 94–96% viability. The results of two separate experiments are shown.

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