Roller bottle cultures yield mature CD83+ DC with high viability. Mature mDC were generated in 850 cm2 roller bottles or in T-175 static flasks as before using ITIP maturation. The floating cells were harvested and stained for DC maturation markers without fixation. Immediately before FACS analysis 2 μg/ml 7-ADD was added as viability indicator. The dot plots shown the total cells in the floating fractions with the CD83+, 7-AAD- and CD83+, 7-AAD+ cells gated. In both cases, the CD83+ cells exhibited 94–96% viability. The results of two separate experiments are shown.