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Table 1 Adherence of peripheral blood CD14+ monocytes to roller bottles and static flasks*

From: A new approach for the large-scale generation of mature dendritic cells from adherent PBMC using roller bottle technology

Condition

CD14+ (%)

CD3+ (%)

CD14- and CD3- (%)

Pre-adherent PBMC

14.6

45.4

40

Flask: Post-adherence

2

59.5

38.4

Roller bottle #1: Post-adherence

2.2

55.2

36.4

Roller bottle #2: Post-adherence

2.1

56

41.9

  1. *PBMC isolated from a normal donor buffy coat donor were incubated in T-175 culture flask or 450 cm2 roller bottles for 2.5 h. The roller bottles were rolled at low speed (1 rpm). The non-adherent cells were isolated and stained along with a sample of the original PBMC for CD14 and CD3 expression. The percent CD14+, CD3+, or CD14-CD3- cells are shown before (pre-adherent PBMC) and after the adherence protocol.
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