Skip to main content

Table 2 Yield of mature DC from roller bottle cultures and static flask cultures*

From: A new approach for the large-scale generation of mature dendritic cells from adherent PBMC using roller bottle technology

Expt # Culture system PBMC seeded per vessel Average floating cells recovered Average mature DC (CD83+, CD86hi) recovered % yield of mature DC
1** 850 cm2 roller bottles 900 × 106 106 × 106 85 × 106 9.4%
  T-175 static flasks 180 × 106 20 × 106 19.2 × 106 10.7%
2** 850 cm2 roller bottles 900 × 106 100 × 106 82 × 106 9.1%
  T-175 static flasks 180 × 106 16 × 106 10.2 × 106 5.7%
3*** 850 cm2 roller bottles 800 × 106 84 × 106 23 × 106 2.9%
  T-175 static flasks 200 × 106 29 × 106 6 × 106 3%
  1. *Human peripheral blood leukopheresis products were seeded and monocytes adhered in the two types of culture vessels, as described in the Methods section. After 4 to 5 days of culture with GM-CSF and IL-4, ITIP cocktail was added to induce DC maturation. On average, 2–3 roller bottles or static flasks were set up for each experiment. The floating cells were harvested one day later and viable cellrecovery was determined by Trypan Blue staining with a hemocytometer followed by FACS staining for CD83 and CD86. The number of mature DC was calculated from the percent CD83+, CD86hi cells using the total number of viable floating cells. The results of three separate experiments are shown.
  2. **Experiment was done directly with normal donor non-G-CSF-mobilized leukopheresis products.
  3. ***Experiment was done directly with a G-CSF-mobilized normal donor leukopheresis product, accounting for the lower yield of mature DC.
\