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Figure 2 | Journal of Immune Based Therapies and Vaccines

Figure 2

From: Use of ultraviolet-light irradiated multiple myeloma cells as immunogens to generate tumor-specific cytolytic T lymphocytes

Figure 2

Effect of UV-irradiation on apoptosis of RPMI 8226 MM cell lines. In order to determine the time of onset of apoptosis of the RPMI 8226 MM cell line following UV-irradiation, tumor cells were first irradiated with 120 mJ/cm2 of UV light and serially (0, 2, 4, 6 and 8 hr post UV-irradiation) analyzed for the onset of early apoptosis (annexin V+PI-) or established apoptosis (annexin V+PI+) using annexin V-FITC/PI dual staining and fluorescence flow cytometric analysis. All experiments were performed triplicate and expressed as mean values.

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