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Figure 4 | Journal of Immune Based Therapies and Vaccines

Figure 4

From: Use of ultraviolet-light irradiated multiple myeloma cells as immunogens to generate tumor-specific cytolytic T lymphocytes

Figure 4

Redirected CTL assay against RPMI following mixed culture conditions with UV-primed RPMI cells. Normal donor PBMCs were primed using UV-irradiated (120 mJ/cm2) or mitomycin-treated (100 μg/mL) RPMI 8226 MM cells as whole cell immunogens for 28 days in 'mixed-culture conditions'. Non-primed, mito-primed, or UV-primed and PBMCs (effector cells) were then co-cultured with viable 3H-TdR-labeled RPMI 8226 MM cells (target cells) in a re-directed CTL assay. A standard 3H-TdR cytotoxicity assays was performed at E:T ratios of 1:1, 3:1, 6:1, 12:1 or 25:1, 50:1 and 100:1.

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