Figure 3

CXCR3, CXCL9, CXCL10, and CXCL11 mRNAs expression by CYP-treated mice. Control or anti-mouse CXCL10 Ab solutions were administered 2 days prior to CYP treatment and every 2 days thereafter. Five days after CYP administration, total RNA was isolated from the spleen, iliac lymph nodes, or urinary bladder of the mice. Panel A: RT-PCR analysis of CXCR3, CXCL9, CXCL10, or CXCL11 mRNA expression was performed. Panel B: RT- PCR analysis of IFN-γ, IL-12 p40, or TNF-α mRNA expression was performed. Log₁₀ copies of transcripts ± SEM are expressed relative to actual copies of 18S rRNA. Asterisks (⋆) indicate statistically significant (p < 0.01) differences between unaffected and CYP-induced groups. Triangles (▲) indicate statistically significant (p < 0.01) differences between control Ab- and anti-CXCL10 Ab-treated groups administered CYP.