Figure 5

Change in NK and NKT cells during acute cystitis. Control or anti-mouse CXCL10 Ab solutions were administered 2 days prior to CYP treatment and every 2 days thereafter. Five days after CYP administration, lymphocytes from the spleen, iliac lymph nodes, and urinary bladder were isolated, stained for DX5 and CD3 expression and analyzed by flow cytometry. Density blots show cell distribution and indicate the percentage of DX5⁺ CD3^- (NK) and DX5⁺ CD3⁺ (NKT) cells, respectively, present in leukocytes isolated from the spleen, iliac lymph node, and urinary bladder.