CXCL10 blockade protects mice from cyclophosphamide-induced cystitis

Table 1 Histological evaluation of CYP-induced cystitis

Treatment/Group	Number of Mice	Cystitis Disease Score (0 – 4)
Control Ab-treated/Naïve	15	0
Anti-CXCL10 Ab-treated/Naïve	15	0
Control Ab treated/CYP-induced	15	3.7 ± 0.4*
Anti-CXCL10 Ab-treated/CYP-induced cystitis	15	1.6 ± 0.6*^,§

The change in urinary bladder inflammation (i.e., cystitis disease score) was evaluated by histopathology. Control or anti-mouse CXCL10 Ab solutions were administered beginning at 2 days prior to CYP (or vehicle alone) treatment and every 2 days thereafter. Five days after CYP (or vehicle alone) administration, mice were sacrificed and urinary bladders were fixed, sectioned at 6 μm, and stained with hematoxylin and eosin. The sections were examined microscopically (at 40× magnification) and scored for the severity of cystitis. The studies were repeated three times, and the data presented are the mean of the cystitis disease score ± standard deviation. Differences between CYP-induced groups and unaffected groups were considered significant when p < 0.01 (*). Differences between control and anti-CXCL10 Ab-treated mice with CYP-induced cystitis were considered significant when p < 0.01 (§).