Evaluation of recombinant invasive, non-pathogenic Eschericia coli as a vaccine vector against the intracellular pathogen, Brucella

Table 4 Immune response gene profile of splenocytes after 5 h immunization with E. coli vaccine.

	Gene	Fold Regulation^a		Gene	Fold Regulation
Th1 Regulation	Csf2	2.07	Th2 Regulation Genes	Il10	1.37
	Ifna4	5.10		Il13	3.14
	Ifng	1.11		Il1f5	-10.20
	Il12b	2.38		Il4	1.04
	Il16	6.73		Il9	9.25
	Il18	-1.04
	Il2	1.27	Other Immune Response Genes	Ifnb1	-3.36
	Il27	-1.11		Il1f10	6.73
	Tnf	3.14		Il1rn	4.44
	Tnfsf15	11.71		Il21	6.28
	Tnfsf4	5.10		Tnfsf8	10.93
	Cd40lg	4.44		Tnfsf9	6.28

^aValues are real-time PCR expression of recombinant invasive E. coli vaccinated animals relative to expression of non-invasive E. coli vaccinated animals. Data were compiled from triplicate wells. T-test p value < 0.001.