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Table 5 Three day cytokine production (pg/ml)a of vaccinated mouse splenic T cells cultured in macrophages with (+) or without (-) 50 μM GFP peptide HYLSTQSAL.

From: Evaluation of recombinant invasive, non-pathogenic Eschericia coli as a vaccine vector against the intracellular pathogen, Brucella

Vaccine Group

Th1 Cytokines

Th2 Cytokines

 

TNFα

IFNγ

IL2

IL4

IL5

 

(-)

(+)

(-)

(+)

(-)

(+)

(-)

(+)

(-)

(+)

GFP

43.3

179.1

421.6

520.8

0

24

3.2

5.2

51.9

41.5

GFPinv

90.7

348.8

628.9

1353.7

1.9

32.2

4.0

3.8

56.2

10.2

GFPinvIL12

79.5

443.7

349.3

1307.2

22.3

36.7

3.2

0

17.9

5.8

()invIL12

110.8

147.5

673.6

862.9

24.8

3.1

3.0

5.9

71.1

41.4

RAW/GFP

134.7

566.2

NAb

NA

10.4

24.8

11.6

0

14.4

0

PBS

127.2

84.5

130.0

440.0

2.2

3

0

12

0

14.1

  1. aValues are from pooled T cells from four mice of each vaccine group. Data are compiled from two experiments. A significant change in expression is indicated by 2-fold or greater over non-peptide treated samples (p < 0.05) and is shown in bold text. Decreases are in italics.
  2. bNot enough cells to perform assay.