Effect of DNA-HSP65 on diabetes incidence and insulitis severity. (A) Diabetes incidence of male mice (6-8 weeks old) that were i.m. injected with three 100-μg doses of DNA-HSP65 or DNAv, given at 15-day intervals, after which they were i.p. injected or not with STZ. Control group mice received i.m. injections of PBS. (B and C) Serum glucose levels were assayed weekly after 5 doses of STZ administration. Additional control groups received only DNA-HSP65 or DNAv. (D) Pancreata of C57BL/6 mice were removed for histological analysis. Each group consisted of 10 mice. To quantify islet infiltration, at least 20 islets from three sections per pancreas were examined in a blinded fashion. Statistical significance was determined by ANOVA followed by Tukey's test. *p < 0.05 and ***p < 0.001 versus control group; #p < 0.05 versus STZ groups.