Figure 4

Influx of CD4⁺ and CD8⁺ cells into the pancreatic islets of STZ-injected mice previously immunized with DNA-HSP65. After three 100-μg doses of DNA-HSP65 or DNAv, given at 15-day intervals, mice were injected with STZ. Control group mice received STZ only (i.p. injections). Pancreatic islets were analyzed 5 weeks after the last STZ injection. Immunostaining for CD4 (A, C, E and G) and CD8 receptors (B, D, F and H). Unspecific staining control (A and B). STZ group mice (C and D), DNA-HSP65+STZ group mice (E and F) and DNAv+STZ group mice (G and H). Quantitative analyses were performed by image processing program (ImageJ). Original magnification, ×20.