HIV-1 neutralization by monoclonal antibody against conserved region 2 and patterns of epitope exposure on the surface of native viruses

Table 2 Neutralization and virus capture (epitope exposure) of 14 HIV-1 isolates by monoclonal antibodies C2EB5, 4E10, 447-52D, and sCD4.

HIV-1 strain	Subtype	IC₅₀(μg/ml)				Virus binding activity³ (p24 antigen (pg/ml))
		C2EB5¹	447-52D	4E10	sCD4	C2EB5	447-52D	sCD4
92RW009	A	36.89	22.5	17.5	8.7	11.33	7.26	6.8
VI191	A	27.1	15	13.75	8.7	11.84	17.78	7.33
QH0692	B	> 50	10.13	> 25	10	3.37	28.59	5.2
SF162	B	> 50	3.7	1.25	7.5	9.72	35.16	8.74
MN	B	> 50	6.25	13.75	9.89	6.25	20.6	5.4
IIIB	B	> 50	8.06	> 25	4.2	2.08	27.69	22.8
92BR025	C	10.01	15	9	10	38.16	5.08	29.44
DU174	C	19.87	1.5	1.25	10	33.13	24.19	32.74
92UG024	D	29.78	21.25	20	6.25	31.54	5.41	29.27
NPO3	AE	12.8	> 25	6.47	3.79	32.47	13.2	29.73
CM244	AE	17.5	21.5	7.5	10	35.55	15.17	28.71
NP1525	AE	12.7	> 25	15	1.87	30.1	2.51	18.68
MENO23²	AE	28.35	> 25	15	10	29.5	4.84	4.66
MENO43²	AE	27.72	> 25	17.3	10	21.62	0	11.23

¹The neutralization activity of C2EB5 at concentrations more than 50 μg/ml was not reported because of cellular toxicity observed above this concentration. ²HIV-1 primary isolate ³The influenza virus was used as negative control virus in each test.