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Table 2 Neutralization and virus capture (epitope exposure) of 14 HIV-1 isolates by monoclonal antibodies C2EB5, 4E10, 447-52D, and sCD4.

From: HIV-1 neutralization by monoclonal antibody against conserved region 2 and patterns of epitope exposure on the surface of native viruses

HIV-1 strain Subtype IC50(μg/ml) Virus binding activity3
(p24 antigen (pg/ml))
   C2EB51 447-52D 4E10 sCD4 C2EB5 447-52D sCD4
92RW009 A 36.89 22.5 17.5 8.7 11.33 7.26 6.8
VI191 A 27.1 15 13.75 8.7 11.84 17.78 7.33
QH0692 B > 50 10.13 > 25 10 3.37 28.59 5.2
SF162 B > 50 3.7 1.25 7.5 9.72 35.16 8.74
MN B > 50 6.25 13.75 9.89 6.25 20.6 5.4
IIIB B > 50 8.06 > 25 4.2 2.08 27.69 22.8
92BR025 C 10.01 15 9 10 38.16 5.08 29.44
DU174 C 19.87 1.5 1.25 10 33.13 24.19 32.74
92UG024 D 29.78 21.25 20 6.25 31.54 5.41 29.27
NPO3 AE 12.8 > 25 6.47 3.79 32.47 13.2 29.73
CM244 AE 17.5 21.5 7.5 10 35.55 15.17 28.71
NP1525 AE 12.7 > 25 15 1.87 30.1 2.51 18.68
MENO232 AE 28.35 > 25 15 10 29.5 4.84 4.66
MENO432 AE 27.72 > 25 17.3 10 21.62 0 11.23
  1. 1The neutralization activity of C2EB5 at concentrations more than 50 μg/ml was not reported because of cellular toxicity observed above this concentration. 2HIV-1 primary isolate 3The influenza virus was used as negative control virus in each test.
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