Kinetics of innate immune cells isolated from the NALT and CLN 5, 24 and 72 h post i.n. immunisation. Cells were isolated from NALT and CLN of Balb/c mice 5, 24 and 72 h after i.n. immunisation with 1 μg LT + 10 μg Ag85B-ESAT6, or PBS (naïve mice) and stained with flurochrome-labelled mAb and analysed by flow cytometry in which 20,000-200,000 events were recorded. (A) Representative dot plots showing CD11c, F4/80 and Ly6G positive populations from both the NALT and CLN of naïve mice. (B) Total cell number is shown for each organ. Columns represent the mean number ± SD. The * indicates significant values of p < 0.05; **, p < 0.01; ***, p < 0.001, as determined by one-way ANOVA followed by Bonferroni's multiple comparison test compared to naïve mice (i.e. 0 h).