Activation status of innate cell populations 5, 24 and 72 h after immunisation. Isolated cells were analysed for MHC II expression, VCAM-1 expression and, CD69 expression. For analysis, gates were set on the innate subset marker positive (CD11c+ or F4/80+) cells and the MFI of MHC II and VCAM-1 expression was determined. A gate was also set around Ly6G+ cells and the level of CD69 expression was determined. Data are presented as mean MFI ± SD with the * indicating significant values of p < 0.05 and **, p < 0.01, as determined by one-way ANOVA followed by Bonferroni's multiple comparison test compared to naïve mice (i.e. 0 h).