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Figure 4 | Journal of Immune Based Therapies and Vaccines

Figure 4

From: Probing local innate immune responses after mucosal immunisation

Figure 4

Immunofluorescent analysis of NALT and CLN early after i.n. immunisation. Both naïve (PBS) and immunised (LT + Ag85B-ESAT6) Balb/c mice were compared. Tissues sections from six individual mice were analysed at 5, 24 and 72 h post immunisation by confocal microscopy. For NALT and CLN, 6 μm frozen sections were stained for (A) CD11c (red), (B) F4/80 (green) or (C) Ly6G (red) and nuclei (blue). A representative picture for each group is shown. There was no staining using isotype control mAb (not depicted). Arrows indicate HEVs; FR, follicular regions; PR, parafollicular regions; E, edges. (Original magnification, ×28.)

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