Figure 5

Differential expression of CAM in the NALT and CLN early after i.n. immunisation. Balb/c NALT and CLN were removed 5, 24 and 72 h post immunisation. Images represent 6 μm serial frozen sections stained for (A) MAdCAM-1, (B) PNAd, (C) ICAM-1 and (D) VCAM-1 (all red) and nuclei (blue). Samples were analysed by confocal microscopy. A representative picture for each group is shown for each staining. There was no staining using isotype control mAb (not depicted). Arrows indicate HEVs; C, cells; MV, micro/blood vessel. (Original magnification, ×28.)