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Table 1 Antibodies used in study.

From: Probing local innate immune responses after mucosal immunisation

Cell Target Target Molecule Clone Isotype Conjugate Source
CAM PNAd MECA 79 IgM none BD Biosciences
CAM MAdCAM-1 MECA 367 IgG2a none BD Biosciences
CAM VCAM-1 MVCAM.A (429) IgG2a none BD Biosciences
CAM ICAM-1 3E2 IgG1 none BD Biosciences
DC CD11c HL3 IgG1 PE-Cy7 or none BD Biosciences
PMN Ly6G RB6-8C5 IgG2b PE or none BD Biosciences
F4/80 C1:A3-1 IgG2b none AbD Serotec
F4/80 BM8 IgG2b TRI-COLOR Invitrogen
PMN CD69 H1.2F3 IgG1 PE or FITC BD Biosciences
DC and MФ I-Ab (MHC II) AF6-120.1 IgG2a FITC BD Biosciences
DC and MФ VCAM-1 MVCAM.A (429) IgG2a Alexa Fluor 647 AbD Serotec
DC, MФ and PMN IFN-γ XMG1.2 IgG1 Alexa Fluor 700 BD Biosciences
DC, MФ and PMN IL-10 JES5-16E3 IgG2b APC BD Biosciences
DC, MФ and PMN TNF MP6-XT22 IgG1 PE-Cy7 BD Biosciences
all cells Hamster Ig none Ig Alexa Fluor 488 or 568 Invitrogen
all cells Rat Ig none Ig Alexa Fluor 488 or 568 Invitrogen
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