Table 1 Antibodies used in study.
From: Probing local innate immune responses after mucosal immunisation
Cell Target | Target Molecule | Clone | Isotype | Conjugate | Source |
|---|---|---|---|---|---|
CAM | PNAd | MECA 79 | IgM | none | BD Biosciences |
CAM | MAdCAM-1 | MECA 367 | IgG2a | none | BD Biosciences |
CAM | VCAM-1 | MVCAM.A (429) | IgG2a | none | BD Biosciences |
CAM | ICAM-1 | 3E2 | IgG1 | none | BD Biosciences |
DC | CD11c | HL3 | IgG1 | PE-Cy7 or none | BD Biosciences |
PMN | Ly6G | RB6-8C5 | IgG2b | PE or none | BD Biosciences |
MФ | F4/80 | C1:A3-1 | IgG2b | none | AbD Serotec |
MФ | F4/80 | BM8 | IgG2b | TRI-COLOR | Invitrogen |
PMN | CD69 | H1.2F3 | IgG1 | PE or FITC | BD Biosciences |
DC and MФ | I-Ab (MHC II) | AF6-120.1 | IgG2a | FITC | BD Biosciences |
DC and MФ | VCAM-1 | MVCAM.A (429) | IgG2a | Alexa Fluor 647 | AbD Serotec |
DC, MФ and PMN | IFN-γ | XMG1.2 | IgG1 | Alexa Fluor 700 | BD Biosciences |
DC, MФ and PMN | IL-10 | JES5-16E3 | IgG2b | APC | BD Biosciences |
DC, MФ and PMN | TNF | MP6-XT22 | IgG1 | PE-Cy7 | BD Biosciences |
all cells | Hamster Ig | none | Ig | Alexa Fluor 488 or 568 | Invitrogen |
all cells | Rat Ig | none | Ig | Alexa Fluor 488 or 568 | Invitrogen |