Figure 1From: Ex vivo development, expansion and in vivoanalysis of a novel lineage of dendritic cells from hematopoietic stem cellsEx vivo HPC to DC expansion and development system. (A) Schematic representation of the LSC culture system and the lentivector constructs. The two LSC lines, LSC-KFT63b and LSC-KFT-GM15, produce the specified hematopoietic growth factors, which support the expansion of HPCs and DCPs. (B) and (C) Semi-quantitative RT-PCR analyses of lentiviral transgene expression in the LSC lines. All of the growth factor genes are human origin except for the mouse GM-CSF (mGM-CSF); the control endogenous mouse GAPDH (mGAPDH) gene expression is shown at bottom.Back to article page