Expansion of CD34+ HPCs and phenotype analysis. (A) Ex vivo expansion kinetics of CD34+ HPCs in LSC culture. HPCs were purified using anti-CD34 Ab magnetic beads and the expansion kinetics on LSC-KFT63b of HPCs from four donors are plotted at bottom. The purified CD34+ cells were analyzed with anti-CD34, anti-CD133, and anti-CD33 Abs by flow cytometry and representative flow graphs of mobilized PB CD34+ HPCs from three donors are illustrated. (B) Flow cytometry analysis of hematopoietic progenitor and differentiation markers after HPC expansion in LSC culture for 9 days. The CD34+ HPCs of cord blood and adult PB were analyzed and presented.