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Figure 4 | Journal of Immune Based Therapies and Vaccines

Figure 4

From: Immunotherapeutic role of Ag85B as an adjunct to antituberculous chemotherapy

Figure 4

Cytokine secretion by PBMCs isolated from untreated/treated tuberculous mice. Two weeks after the treatment, PBMCs were obtained from the untreated/treated tuberculous mice (5 animals per group) and in vitro stimulated with or without rAg85B in quintuplicates. Supernatants were collected from the cultures, and the levels of respective cytokines were measured by ELISA assay. The results are expressed as mean ± standard deviation of five OD values after deducting the values of unstimulated wells. Statistical analysis of the results was carried out by Student's t test. The basal levels of cytokines were estimated two weeks after infection with M. tuberculosis, just before the treatment. (ATD: Antituberculous Drugs; IT: Immunotherapy; O/W: Once Weekly)

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