Figure 1

Quantification of acute lung epithelial injury. The efflux of alveolar protein tracer (¹³¹I-albumin) from lungs to the circulation was calculated in 4-h experiments of rats as an index of acute lung epithelial injury. In the control group (Control, no bacteria), only lactated Ringer's solution was instilled into the airspace of the rats and no therapeutic intervention was taken. Three sets of rats were co-instilled P. aeruginosa PA103 with 4 mg/kg of either irrelevant monoclonal IgG (Control IgG), rabbit polyclonal anti-PcrV IgG (Rab anti-PcrV), or murine monoclonal anti-PcrV IgG (Mab166). Four sets of rats were intratracheally administered either PBS, rabbit polyclonal anti-PcrV IgG (Rab anti-PcrV)(4 mg/kg), murine monoclonal anti-PcrV IgG (Mab166)(4 mg/kg), or Mab166 Fab (4 mg/kg). Data are shown as means+standard errors. The numbers of rats are listed in Table 1. +P < 0.05 to the control group (no bacteria) and *P < 0.05 to the control IgG group in co-instillation and to the group without IgG (PBS) in therapeutic administration by two way-ANOVA, repeated measure, followed by the Newman-Keuls t-test.