Table 3 Immuno-reactivity of biotinylated HIV peptides derived from different clades using mice antisera against V3-gp41, gp41 and p17 peptides. Immunoreactivity of biotinylated HIV peptides using mice antisera raised against different HIV peptides. Titer of antisera was defined as the reciprocal of dilution which gave a mean absorbance of 0.5. Run was considered invalid if negative control gave a mean absorbance > 0.5.

Clade A

NTRKSV-RIGPGQAF-Y

3.00

1.20

0.40

0.08

1348

Clade B

NTRKSI-HIGPGRAF-Y

3.00

1.35

0.37

0.13

1318

Clade C

NTRKSI-RIGPGQTF-Y

3.00

1.27

0.28

0.09

1148

Clade D

NTRQRT-HIGPGQAL-Y

2.89

1.16

0.28

0.10

1122

Clade E

NTRTSI-TRGPGQVF-Y

3.00

1.25

0.36

0.10

1258

Clade F

NTRKSI-HLGPGQAF-Y

2.90

1.07

0.32

0.13

1148

Clade G

NTRKSI-TIGPGQAF-Y

2.89

1.05

0.26

0.19

1023

MN Bio

NKRKRI-HIGPGRAF-Y

2.90

0.91

0.24

0.12

933

SF2 Bio

NTRKSI-YIGPGRAF-H

2.87

1.02

0.35

0.15

1148

BR1

NTRKSI-HIGWGRAF-Y

2.80

0.98

0.30

0.15

1047

BR2

NTRKSI-HMGW-GRAF-Y

2.64

0.96

0.28

0.16

1023

p17

YSVHQRIDIKETKDALEKIDDDNKSKKA

2.90

1.50

1.10

0.65

7000

gp41

DRPEGIEEEGERRDRDTS

2.90

2.80

1.80

1.0

7500

No Peptide

-

0.12

0.05

0.06

0.04

11