Generating neutralizing antibodies, Th1 response and MHC non restricted immunogenicity of HIV-I env and gagpeptides in liposomes and ISCOMs with in-built adjuvanticity

Agrawal, Lokesh; Haq, W; Hanson, Carl Veith; Rao, D Nageswara

doi:10.1186/1476-8518-1-5

Journal of Immune Based Therapies and Vaccines

Table 4 Cytokine levels for various peptides in different haplotypes. Cytokine levels as measured for various peptide formulations in different haplotypes. The value represents the level of cytokines obtained from wells pulsed with the optimal antigen (peptides alone or in adjuvant systems) concentration which showed maximal T-cell stimulation index).

From: Generating neutralizing antibodies, Th1 response and MHC non restricted immunogenicity of HIV-I env and gagpeptides in liposomes and ISCOMs with in-built adjuvanticity

Strain→	H-2 Haplotypes				Cytokines (pg/ml)
Formulation↓	BALB/c (H-2^d)	CBA/J (H-2^k)	C57BL/6J (H-2^b)	FVB/J (H-2^q)
V3-gp41 Peptide	611 ± 21	311 ± 35	727 ± 68	878 ± 240	IFN-γ
	372 ± 90	342 ± 28	2322 ± 245	260 ± 54	IL-2
	29 ± 3	9.0 ± 1	35 ± 3	42 ± 6	IL-4
V3-gp41 in Liposomes	1076 ± 102	855 ± 44	1332 ± 221	1854 ± 132	IFN-γ
	367 ± 35	408 ± 36	443 ± 24	475 ± 25	IL-2
	19 ± 2	8.0 ± 2	40 ± 5	7.0 ± 1	IL-4
V3-gp41 in ISCOMs	1956 ± 145	2076 ± 126	2178 ± 231	2589 ± 321	IFN-γ
	665 ± 42	757 ± 60	667 ± 32	660 ± 35	IL-2
	12 ± 1	12 ± 2	36 ± 5	4.0 ± 1	IL-4
V3-gp41: MA729 in Liposomes	1856 ± 121	2325 ± 231	2560 ± 265	2667 ± 320	IFN-γ
	821 ± 69	754 ± 58	702 ± 36	753 ± 36	IL-2
	15 ± 5	13 ± 3	29 ± 5	18 ± 5	IL-4
gp41 Peptide	520 ± 56	655 ± 33	765 ± 36	333 ± 26	IFN-γ
	200 ± 12	398 ± 25	390 ± 25	432 ± 20	IL-2
	53 ± 5	32 ± 3	57 ± 6	41 ± 6	IL-4
gp41 Peptide in Liposomes	989 ± 65	1177 ± 125	1098 ± 89	980 ± 66	IFN-γ
	445 ± 25	506 ± 55	535 ± 21	582 ± 32	IL-2
	25 ± 6	15 ± 3	45 ± 5	7.0 ± 1	IL-4
gp41 Peptide in ISCOMs	1884 ± 155	1765 ± 211	1678 ± 201	2345 ± 315	IFN-γ
	700 ± 36	814 ± 54	721 ± 58	765 ± 75	IL-2
	15 ± 4	46 ± 5	52 ± 6	24 ± 3	IL-4
gp41 : MA729 in Liposomes	2577 ± 220	2436 ± 233	2245 ± 165	2100 ± 103	IFN-γ
	789 ± 100	676 ± 20	723 ± 65	703 ± 87	IL-2
	45 ± 6	32 ± 5	27 ± 8	40 ± 5	IL-4
p17 Peptide	433 ± 21	623 ± 12	453 ± 52	534 ± 31	IFN-γ
	360 ± 21	183 ± 24	243 ± 34	253 ± 50	IL-2
	20 ± 6	37 ± 3	29 ± 8	23 ± 6	IL-4
p17 Peptide in Liposomes	350 ± 30	786 ± 35	998 ± 53	1052 ± 32	IFN-γ
	450 ± 27	387 ± 26	427 ± 32	364 ± 32	IL-2
	30 ± 10	20 ± 4	51 ± 3	45 ± 6	IL-4
p17 Peptide in ISCOMs	677 ± 59	1321 ± 136	1200 ± 70	1400 ± 230	IFN-γ
	618 ± 60	345 ± 32	627 ± 51	501 ± 34	IL-2
	23 ± 4	23 ± 4	12 ± 4	57 ± 5	IL-4
p17 : MA729 in Liposomes	1756 ± 100	1899 ± 120	1500 ± 52	1876 ± 320	IFN-γ
	489 ± 52	665 ± 50	603 ± 35	581 ± 18	IL-2
	44 ± 13	41 ± 10	36 ± 5	39 ± 6	IL-4
p24 Peptide	435 ± 23	654 ± 36	500 ± 35	435 ± 35	IFN-γ
	354 ± 29	385 ± 25	256 ± 23	334 ± 26	IL-2
	18 ± 5	10 ± 2	34 ± 5	21 ± 8	IL-4
p24 Peptide in Liposomes	975 ± 63	987 ± 54	1123 ± 124	786 ± 69	IFN-γ
	470 ± 56	235 ± 19	398 ± 54	402 ± 30	IL-2
	29 ± 6	30 ± 3	22 ± 1	32 ± 4	IL-4
p24 Peptide in ISCOMs	1352 ± 69	1354 ± 54	1245 ± 103	1532 ± 301	IFN-γ
	665 ± 80	478 ± 22	444 ± 31	676 ± 88	IL-2
	13 ± 5	8.0 ± 6	19 ± 4	28 ± 4	IL-4
p24 : MA729 in Liposomes	1543 ± 56	1763 ± 240	1324 ± 99	1432 ± 26	IFN-γ
	556 ± 40	600 ± 56	655 ± 87	543 ± 45	IL-2
	23 ± 6	27 ± 4	38 ± 8	21 ± 7	IL-4

The value represents the level of cytokines obtained from wells pulsed with the optimal antigen (peptides alone or in adjuvant systems) concentration which showed maximal T-cell stimulation index.

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